Universitat Autònoma de Barcelona
Cerdanyola del Vallès
Translational Research Imaging Center
Department of Clinical Radiology
University Hospital Münster/DE
Ana Paula Candiota
Centro de Investigación Biomédica en Red
Universitat Autònoma de Barcelona
Cerdanyola del Vallès, Barcelona/ES
A. P. Candiota, C. Faber, M. Julià-Sapé, N. Just, F. Schmid, L. Wachsmuth
Goals of the course:
The course on Small animal MR imaging will address basic technical and practical aspects of MRI with emphasis on demands for small animal application. The lectures will provide basic knowledge about the major aspects of appropriate animal handling, anaesthesia and of monitoring and maintaining a stable physiological state during imaging. Specifically, sensitivity issues and the impact of physiological motion will be addressed. Different strategies to deal with respiratory and heart motion will be introduced. The course will introduce requirements and methods of small animal MR spectroscopy and give an overview of preclinical applications of MRS. MR methods for neuroimaging and functional MRI will be introduced in detail and novel approaches such as optogenetic fMRI will be discussed in detail.
This course will emphasize
• Small animal physiology, gating techniques, tissue processing and preparation
• Basic principles and methods of MRS
• Introduction of advanced neuroimaging MR methods such as BOLD, MEMRI, DWI and DTI
• General overview of preclinical MR applications
• Introduction of optogenetic fMRI and multimodal functional neuroimaging
The course is intended for scientists and students with a strong interest in small animal imaging. A background in MRI is helpful but not mandatory. The lectures will focus on applications and practical issues. The major aim of the course is to detail the particular requirements for small animal MRI and MRS, including maintaining stable physiological conditions and advanced MRI and MRS techniques for preclinical imaging. The course will further introduce multimodal neuroimaging including optogenentics.
Small Animal MR imaging has come to play a key role in biomedical research for studying experimental rodent models of human diseases. Because of its non-invasiveness and versatility, the non-invasive MR technique complements biochemical and histochemical methods for investigating biological processes under normal and pathological conditions.
The course will address basic technical and practical aspects of MRI/MRS/MRSI with emphasis on demands for small animal application. The major aspects of appropriate animal handling, anaesthesia and of monitoring and maintaining a stable physiological state during imaging will be explained. Specifically, sensitivity issues and the impact of physiological motion will be addressed. Different strategies to deal with respiratory and heart motion will be introduced.
One focus will be the particular requirements and methods for MR spectroscopy in rodents. Selected presentations will provide more detailed information about advanced MR methods and their preclinical applications. Reviews from different fields of neuroimaging will illustrate that MRI and MRS can not only provide qualitative anatomical information but permit reliable measurements of quantitative parameters directly related to the neurophysiological state, to tissue integrity and structure, and even to functional aspects.
Finally, a session on optogenetic fMRI will introduce this novel field and provide practical details on how to perform optical stimulation and recordings in the MR scanner.
The course is intended for students, scientists and clinicians with a strong interest in biomedical applications of small animal imaging. Prior knowledge of basic MR will be helpful although non-specialists in MR are welcome to register.
Learning objectives: MR basics
•Introduction to MRI and MRS
•Contrast mechanisms and MRI techniques (BOLD, Diffusion, MT)
•Contrast agents (T1-, T2* agents)
•Hardware (RF coil design, including array coils and cryo probes)
Small animal imaging
•Rodent physiology and monitoring (animal handling, procedures, anaesthesia)
•Prospective and retrospective gating
•Tissue processing, ex vivo sample preparation